Let $\mathscr{F}$ be a pre sheaf on $X$, let $\mathscr{F}^+$ be a sheaf on $X$, and let $\varphi:\mathscr{F}\to\mathscr{F}^+$ be a morphism of presheaves such that for all $x\in X$, $\varphi_x$ is an isomorphism. Does this imply that $\mathscr{F}^+$ is the sheafification of $\mathscr{F}$?
Clearly, if $\varphi:\mathscr{F}\to\mathscr{F}^+$ is already the sheafification, then this property is satisfied, but it seems easier to test for the first property than to try using universal properties.