Whenever you apply a spectral projection $E(S)\ne I$ to $A$, you end up with $0$ in the point spectrum of $E(S)A=AE(S)$ because $\{AE(S)\}E(\sigma\setminus S)=0$ and $E(\sigma\setminus S) \ne 0$. So that special case always requires special attention.
If $(a,b)\subseteq\sigma(A)$, then $E(a,b) \ne 0$; otherwise $\mu \in (a,b)$ would lead to a bounded operator,
$$
R(\mu)=\int_{\sigma}\frac{1}{\lambda-\mu}dE(\mu),
$$
which would have to be the resolvent $R(\mu)=(A-\mu I)^{-1}$. And that would force $(a,b)\in\rho(A)$, contrary to assumption.
Suppose $[0,1]\subseteq\sigma(A)$. Then $E(a,b) \ne 0$ for $(a,b)\subseteq[0,1]$. I'll first consider your first question where you ask about the spectrum of $AE[1/4,1/2)$. Automatically $0\in\sigma(AE[1/4,1/2))$ because $\{AE[1/4,1/2)\}E(1/2,1)=0$ and $E(1/2,1)\ne 0$. For $\mu\ne 0$ and $\mu\notin [1/4,1/2]$,
$$
AE[1/4,1/2)-\mu I=(A-\mu I)E[1/4,1/2)-\mu E(\sigma\setminus[1/4,1/2))
$$
has a bounded inverse given by
$$
(AE[1/4,1/2)-\mu I)^{-1}=\int_{[1/4,1/2)}\frac{1}{\lambda-\mu}dE(\lambda)-\frac{1}{\mu}E(\sigma\setminus[1/4,1/2)).
$$
Therefore, $\sigma(AE[1/4,1/2))\subseteq [1/4,1/2]\cup\{0\}$. Conversely $0\in\sigma(AE[1/4,1/2))$ was noted above, and, for any $\mu\in(1/4,1/2)$, the projections $E(\mu-\delta,\mu+\delta)\ne 0$ for all $\delta > 0$, which gives the existence of a non-zero vector $x_{\delta}$ such that $E(\mu-\delta,\mu+\delta)x_{\delta}=x_{\delta}$ and, hence,
\begin{align}
\|AE[1/4,1/2)x_{\delta}-\mu x_{\delta}\|
& = \|(A-\mu I)E(\mu-\delta,\mu+\delta)x_{\delta}\| \\
& \le \delta \|E(\mu-\delta,\mu+\delta)x_{\delta}\| \\
& = \delta\|x_{\delta}\|.
\end{align}
So $AE[1/4,1/2)-\mu I$ cannot be continuously invertible, which proves
$$
\{0\}\cup (1/4,1/2) \subseteq \sigma(AE[1/4,1/2))
$$
Because the spectrum is closed,
$$
\{0\} \cup [1/4,1/2]\subseteq \sigma(AE[1/4,1/2)).
$$
The opposite inclusion was previously shown. So
$$
\sigma(AE[1/4,1/2))=\{0\}\cup[1/4,1/2].
$$
The operator $AE[1/4,1/2]$ is selfadjoint. So its norm is its spectral radius, which gives $\|AE[1/4,1/2)\|=1/2$.
I'll let you consider the other cases. Note for example that $S=[1/3,1/2]\cap\mathbb{Q}$ could be such that $E(S)=0$, or it could give $E(S)=E[1/3,1/2]$, or $E(S)=E(T)$ could hold for a lot of closed subsets $T$ of $[1/3,1/2]$ because the spectrum is closed, and every subset of $[1/3,1/2]\cap\mathbb{Q}$ could consist of eigenvalues.