There is a nice generalization of this fact due to John Thompson, known as the "Thompson transfer Lemma". It goes as follows: let $G$ be a finite group which has a subgroup $M$ such that $[G:M] = 2d$ for some odd integer $d$, and suppose that $G$ has no factor group of order $2$. Then every element of order $2$ in $G$ is conjugate to an element of $M$. I will not give the full proof as it reveals too much of the solution of the original question, but the idea is the same: any element of order $2$ in $G$ which does not lie in any conjugate of $M$ must act as an odd permutation in the permutation action of $G$ of the (say, right) cosets of $M$. As a sample application, consider a finite non-Abelian simple group $G$ whose Sylow $2$-subgroup $S$ has a cyclic subgroup $M$ of index $2$. Then $G$ certainly has no factor group of order $2$, so every element of order $2$ (involution) of $G$ is conjugate to an involution of $M$. But $M$ only has one involution as $M$ is cyclic, so $G$ has one conjugacy class of involutions. In case anyone is wondering, the Thompson Transfer Lemma is a true generalzation of the question, because the case $M = 1$ can be applied to the question to conclude that there must be a normal subgroup of index $2$ for $G$, because no element of order $2$ lies in any conjugate of the trivial group.